Rhizoma Drynarinae Extract Powder

Our team pay attention to foreign trade internet marketing outsourcing service

 

The extract of Drynaria fortunei (Kunze) J.SM is a dry rhizome extract of the plant Drynaria fortunei (Kunze) J.SM in the family Polypodiaceae. Its main active substance is naringin, and it also contains various chemical substances such as methyleugenol, protocatechuic acid, New Beimei Shengcao glycoside, dihydroflavonoid glycoside of Drynaria fortunei, cyclosterol acetate, cycloopium sterol acetate, and rapeseed sterol. It has functions such as promoting proliferation and differentiation, anti osteoporosis, anti-inflammatory, promoting fracture healing, protecting dental bone cells, protecting kidneys, preventing drug-induced hearing loss, and lowering blood lipids. The methanol hydrate extract of Bone Fragment Bu has inhibitory activity on elastase and can be used in cosmetics and drugs to treat skin aging, allergies, inflammation, pigment deposition, etc.

 熊果苷依结构不同可分为α型和β型。α-熊果苷化学名为 4-羟基苯基-α-D-吡喃葡萄糖苷，β-熊果苷化学名为4-羟基苯基-β-D-吡喃葡萄糖苷。α-熊果苷为β-熊果苷的差向异构体，其糖苷键在空间上的方向与β-熊果苷相反。

 

 

Chemical Composition

 

1. Flavonoids and their glycosides: These compounds are mainly flavonoid glycosides with kaempferol and luteolin as aglycones. Dihydroflavones and their glycosides: The main components of dihydroflavones in Rhizoma Drynariae are North American resveratrol, naringenin, and matrine and their glycosides. Naringin is an important active ingredient in Rhizoma Drynariae. Xanthanols and their glycosides: Compounds mainly composed of catechin, aflatechin, and epicatechin, as well as Xanthanol glycosides with epicatechin as the aglycone, were isolated from Bone Fragment Bu.

2. Triterpene compounds are mostly obtained from mineral oil ether extracts of Polygonum multiflorum, such as sheep tooth - (11) - ene, resveratrol, cyclo - (29) - aldehyde, cyclo - (11) - alcohol, resveratrol, hopa-21-ene, northeast guanzhong alcohol, hopa-22 (29) - ene, and Chiratone.

3. Phenylpropanoid compounds were isolated from Fructus Aurantii to obtain ferulic acid - β - D-glucopyranoside (E) -4-O-β - D-glucopyranosylcaffeic acid, coumaric acid -4-O-β - D-glucopyranoside, transcaffeic acid sodium, dihydroisoferulic acid, dihydrocaffeic acid, and (E) - p-pine needle acid - β - B-glucopyranoside.

4. Phenolic acids and phenolic acids. The phenolic acid components contained in bone shreds of Bu are mainly benzoic acid and phenylpropanoic acid, among which phenylpropanoic acid compounds are mainly composed of cinnamic acid, ferulic acid, and caffeic acid as aglycones.

5. Lignin and steroids were isolated from bone fragments, and lignin compounds such as larch resin 4c-O-B-D-glucopyranoside, (7cR, 8cS) - dihydrodehydrodipine base alcohol 4c-O-B-D-glucoside, as well as steroid compounds such as B-sitosterol and β - carotene were obtained.

6. Other effective ingredients: 30% ethanol elution, separate protocatechuic acid and vanillin-4-O-β - D-glucopyranoside from bone fragments; 50% ethanol extract resin -30% ethanol Chemicalbook elution, separation of 3-acetamide-4-hydroxybenzoic acid and 5-ethyl-2-hydroxybenzoate ethyl ester from fragmented bone. (1) Diploptene: Molecular formula C30H50, molecular weight 410.3954. Colorless needle crystal (ether), mp.208 ℃~211 ℃. (2) Hop-21 ene: Molecular formula C30H50, molecular weight 410.3818. Colorless crystalline flakes (ether), mp.168 ℃~169 ℃. (3) Diploptenol: Molecular formula C30H52O, molecular weight 428.4026. Colorless crystalline flakes (ether), mp.242.5 ℃~244 ℃. (4) Fern-9 (11) ene: Molecular formula C30H50, molecular weight 410.3891. Colorless needle crystal (ether), mp.171 ℃~173 ℃. (5) Cyclolaudenol: also known as cyclic opioid sterol. Molecular formula C31H52O. Molecular weight 440.3976. Colorless crystalline flakes (acetone), mp.137 ℃~138.5 ℃. (6) Cyclomargenol: Molecular formula C32H54O, molecular weight 454.4134. Colorless crystalline flakes (acetone), MP-110 ℃ to 113 ℃. (7) Cyclolaudenone: Synonymous with cyclic opioid sterone. Molecular formula C31H50O, molecular weight 438.3841. Colorless crystalline flakes (ether), MP.108 ℃ to 112 ℃. (8) N-Dotriacontanicid: Molecular formula C32H64O2, molecular weight 480.4753. White powder, MP.54.0 ℃~55.5 ℃.

Specifications：

Pharmacological Action：

1. The effect of lowering blood lipids: The intramuscular injection of the alcohol extract of Drynaria fortunei can prevent the increase of serum cholesterol and triglycerides in rabbits caused by high-fat feed, reduce the increase, prevent the formation of atherosclerotic plaques in the aorta, and significantly reduce cholesterol in the liver and adrenal glands. Drynaria fortunei polysaccharide acid salt also has significant anti-hyperlipidemia and anti-atherosclerosis effects on rabbits, and can promote the recovery of damage to mitochondria and rough endoplasmic reticulum in liver and adrenal cells caused by hypercholesterolemia, thereby promoting the conversion and excretion of cholesterol in liver and adrenal cells. Dihydroflavonoid glycosides also have a tendency to reduce serum cholesterol and triglycerides.

2. Promote fracture healing. The extract of Drynaria fortunei can promote the absorption of calcium by the bone and increase the levels of blood calcium and phosphorus, which is beneficial for fracture healing. Taking the pill of Bone-Healing II (mainly containing Drynaria fortunei) orally to rabbits with artificial fractures can accelerate the absorption, organization, callus growth, and remodeling of hematomas, and promote the growth of endosteum, especially active osteoblast activity. For experimental arthritis in young rats, treatment with Drynaria fortunei decoction can improve the function of cartilage cells, delay cell degeneration, and reduce the incidence of bone and joint disease.

3. Reducing the toxicity of antibiotics: When guinea pigs were injected with streptomycin 600mg/kg intraperitoneally, all five guinea pigs in one group that were not given Drynaria fortunei died. In the other group of seven guinea pigs, only two died after being given a decoction of Drynaria fortunei (equivalent to 100g/kg of crude drug) before injection. This indicates that Drynaria fortunei can significantly reduce the mortality rate of animals caused by streptomycin toxicity. In addition, Drynaria fortunei can also significantly reduce the damage to the ear caused by kanamycin.

4. The effect on the cardiovascular system: The dihydroflavonoid glycosides isolated from Drynaria fortunei have a strong cardiac effect. When administered intravenously to the ear vein of rabbits in a 0.5% solution, the cardiac effect lasts for 2 hours, enhancing myocardial contractility and regularizing heart rhythm. There is no significant effect on heart rate and blood pressure. Its cardiac effect is directly on the heart muscle rather than on the sympathetic nervous system. Drynaria fortunei also enhances the ability of mice to endure hypoxia and reduces platelet aggregation in rabbits.

5. Sedative and Analgesic Effects: The dihydroflavonoid glycosides in Drynaria fortunei exhibit notable sedative and analgesic effects.

6. Antibacterial effect: The extract of Drynaria fortunei can inhibit the growth of staphylococcus in vitro.

Extraction and Separation:

【注1】Add ethanol to 70% alcohol content, and let it stand at 5 ℃ to 10 ℃ for 24 hours to remove the paste like precipitate. The solution is then washed in a water bath to evaporate the ethanol and concentrated to the appropriate consistency. Add ethanol to 90% alcohol content, and let it stand at 5 ℃ to 10 ℃ for 24 hours.
【注2】 Boil the filtrate with 2% activated carbon in a water bath for decolorization, filter, evaporate ethanol in the water bath, dilute with water, and extract three times with saturated n-butanol.

（Note：Separation of lipophilic components）

Methods of Detection:

1. Physical and chemical identification
Take 0.5g of powder, add 30ml of methanol, heat and reflux for 1 hour, cool, filter, evaporate the filtrate, and dissolve the residue in 1ml of methanol as the test solution. Take another methanol solution of naringin reference substance (0.5mg/ml). Take 4ml of each of the above two solutions and place them on the same silica gel G thin layer plate. Develop an upper layer solution of benzene ethyl acetate formic acid water (1:12:2.5:3), spray with aluminum trichloride Chemicalbook test solution, and examine under a UV lamp (365nm). The test sample chromatography shows fluorescent spots of the same color at the corresponding positions as the reference sample chromatography.

2. UV spectroscopic identification
Take 1g of crude powder from the sample and place it in a Soxhlet extractor. Extract with 80ml of 95% ethanol and reflux for 12 hours. Recycle the ethanol to dryness, dissolve the residue in methanol and make up to 100ml. Measure its UV spectrum. As a result, there was a maximum absorption peak in the wavelength range of 281nm for the bone fragment patch.

Certificates：

Package：

5 Grams to 2kg Packaging: Alu-bag	5 KG/Al-Tin Packaging: 2 pcs Alu-tin with box	25 KG, Packaging: Drum (54*37*37cm)=0.07CBM N.W:25KG / G.W:28KG

Delivery：

Contact Us

Whether you'd like to get more info on what, why and how we do what we do, need help with what you're
creating, would like to partner up or just say hi! ---we'd love to hear from you! 

FAX

+86-29-8832 8052

E-mail

sales@greenspringbio.com

SKYPE

hellenhu2

Whatsapp

+86 132 2777 5215